Annual reports on fermentation processes Volume 6 by George T Tsao; Michael C Flickinger; Robert K Finn

By George T Tsao; Michael C Flickinger; Robert K Finn

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Extra resources for Annual reports on fermentation processes Volume 6

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TOLBERT AND JOSEPH FEDER Table 1. 3° Rat Osteosarcoma Medulloblastoma Careinosarcoma Cells grew as small suspension. aggregate clumps or as single cells b in b Representative yields cells from at least Corresponds Reprinted to ca. , from volume of wet of culture. eel Is/ml for this packed line. (32). waste is pumped into a steam-heated kill tank. These 100-liter vessels have been extensively used over the past few years in production of large volumes of suspension culture. Table 1 lists several continuous mammalian cell lines and representative cell yields obtained in this system.

Molecules which require post-translational processing,glycoproteins and lipoproteins presently are not amenable to biosynthesis via recombinant methods. Large-scale cell culture processes are necessary for the production of such molecules. Often biological activity is represented by several distinct molecular ANNUAL REPORTS ON FERMENTATION PROCESSES. VOL. 6 35 Copyright © 1983 by Academic Press, Inc. All rights of reproduction in any form reserved. ISBN 0-12-040306-4 36 WILLIAM R. TOLBERT AND JOSEPH FEDER forms, all of which may be concurrently produced in a single vertebrate culture system as in interferon production.

They demonstrated that the process was effective in the temperature range of 10° through 35°C, and the system was remarkably stable and resistant to shock loadings. Although two pumps are utilized in this system, one for expanding the bed and the other to deliver the feed, the energy requirement is relatively modest since the flow required to achieve a 20% expansion of the bed does not require a major input of energy. The advantage of expanding the bed appears to be the elimination of clogging problems while accumulating significant quantities of cells on the surface of the particles.

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